Journal article
Mineralisation of 2,6-dichlorobenzamide (BAM) in dichlobenil-exposed soils and isolation of a BAM-mineralising Aminobacter sp
Department of Geochemistry, Geological Survey of Denmark and Greenland (GEUS), Øster Voldgade 10, DK-1350 Copenhagen K, Denmark.1
Mineralisation of the groundwater contaminant 2,6-dichlorobenzamide (BAM), a metabolite from the herbicide 2,6-dichlorobenzonitrile (dichlobenil), was studied in soil samples obtained from 39 locations previously exposed to dichlobenil. Rapid BAM mineralisation was detected in samples from six locations with 5.2-64.6% of the added BAM mineralised within 48-50 days.
From one location rapid BAM mineralisation was observed in soil samples down to a depth of 2 m below the surface. One location with fast BAM mineralisation showed significant dichlobenil degradation activity with 25.5% of the added dichlobenil being mineralised within 50 days. By inoculating soil showing the fastest mineralisation of BAM into a mineral medium with BAM as the only carbon and nitrogen source an enrichment culture was established.
Community analysis based on extracted DNA revealed a change of the bacterial community but without any clear indication of key members within the BAM-mineralising culture. Parallel cultivation resulted for the first time in the isolation of a BAM-mineralising bacterium, identified as an Aminobacter sp.
Language: | English |
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Year: | 2006 |
Pages: | 289-295 |
ISSN: | 02697491 and 18736424 |
Types: | Journal article |
DOI: | 10.1016/j.envpol.2005.11.047 |
ORCIDs: | Sørensen, Søren J |
2,6-Dichlorobenzamide 2,6-Dichlorobenzonitrile 3',4'-dichlorobenzamil Amiloride Aminobacter sp. Base Sequence Biodegradation, Environmental Electrophoresis, Polyacrylamide Gel Genes, Bacterial Herbicide Herbicides Mineralisation Molecular Sequence Data Nitriles Ribotyping Soil Microbiology Soil Pollutants dichlobanil