Journal article
Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia▿ †
U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland,1
The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA).
All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.
| Language: | Undetermined |
|---|---|
| Publisher: | American Society for Microbiology (ASM) |
| Year: | 2010 |
| Pages: | 1996-2001 |
| ISSN: | 10985336 and 00992240 |
| Types: | Journal article |
| DOI: | 10.1128/AEM.01919-09 |
