Journal article
Effects of 3'-deoxy-3'-fluorothymidine on proliferation kinetics of Ehrlich ascites tumour cells in vitro (comparison with other cytostatic drugs)
Central Institute for Molecular Biology, Berlin, German Democratic Republic
Central Institute for Cancer Research of the Academy of Sciences of the German Democratic Republic, Berlin, German Democratic Republic
Treatment of Ehrlich ascites carcinoma cells in vitro with cytosine-arabinoside, 5-fluoro-2′-deoxyuridine and hydroxyurea in concentrations just completely inhibiting cell proliferation, results, in agreement with data published for other cells, in progressive loss of viability if the treatment exceeds a period of one generation time (about 14hr).
Pulse cytophotometric analysis of the treated cells showed them to be located mainly in the G1-phase of the cell cycle. In contrast, cell proliferation can be inhibited for about 32hr (more than 2 generation times), without loss of viability, if the treatment is done with 3′-deoxy-3′-fluorothymidine (3′-FTdR).
Pulse cytophotometric analysis revealed a concentration-dependent delay of progression through the S-phase. At a concentration just completely inhibiting cell proliferation, discontinuation of proliferation was predominant in late S-phase at the G2-boundary. These properties recommend 3′-FTdR for experiments aimed at improving cancer chemotherapy by a non-toxic accumulation of cells in the S-phase followed by treatment with a second, S-phase specific agent.
Language: | English |
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Year: | 1978 |
Pages: | 349-354 |
ISSN: | 18792995 and 00142964 |
Types: | Journal article |
DOI: | 10.1016/0014-2964(78)90204-9 |