Journal article
Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
Department of Pharmaceutical Chemistry, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA1
Center for Brain Science Howard Hughes Medical Institute, Harvard University, Cambridge, MA 021382
Department of Chemistry and Chemical Biology and Department of Physics Howard Hughes Medical Institute, Harvard University, Cambridge, MA 021383
Anyone who has used a light microscope has wished that its resolution could be a little better. Now, after centuries of gradual improvements, fluorescence microscopy has made a quantum leap in its resolving power due, in large part, to advancements over the past several years in a new area of research called super-resolution fluorescence microscopy.
In this Primer, we explain the principles of various super-resolution approaches, such as STED, (S)SIM, and STORM/(F)PALM. Then, we describe recent applications of super-resolution microscopy in cells, which demonstrate how these approaches are beginning to provide new insights into cell biology, microbiology, and neurobiology.
| Language: | English |
|---|---|
| Year: | 2012 |
| Pages: | 1047-1058 |
| ISSN: | 10974172 and 00928674 |
| Types: | Journal article |
| DOI: | 10.1016/j.cell.2010.12.002 |
