Journal article
Lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk
INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. acfernandes@inesc-mn.pt.1
INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. admargduarte@fmv.ulisboa.pt.2
INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. fcardoso@inesc-mn.pt.3
CIISA, Veterinary Medicine Faculty, Technical University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisbon, Portugal. ricardobexiga@fmv.ulisboa.pt.4
INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. scardoso@inesc-mn.pt.5
INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. pfreitas@inesc-mn.pt.6
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells.
This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk.
Language: | English |
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Publisher: | MDPI |
Year: | 2014 |
Pages: | 15496-15524 |
ISSN: | 14243210 and 14248220 |
Types: | Journal article |
DOI: | 10.3390/s140815496 |