Journal article
Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5
Instituto de Bioquímica Médica, UFRJ, CCS, Bloco D, subssolo 21491-590 Rio de Janeiro, Brazil1
Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, UERJ, Blv 28 de Setembro, 87, fundos, 4 andar, Vila Isabel, Rio de Janeiro, RJ 20551-013, Brazil2
Laboratório de Tecnologia em Bioquímica e Microscopia, Centro Universitário Estadual da Zona Oeste, Rio de Janeiro, 23070-200, Brazil3
Embrapa Agrobiologia BR465, Km 07 Seropédica Rio de Janeiro, 23851-970, Brazil4
Instituto de Biologia, Departamento de Entomologia e Fitopatologia, Universidade Federal Rural do Rio de Janeiro Cx Postal 74585/BR 465, KM 07, Seropédica, RJ 23851-970, Brazil5
Lab. Biotecnologia- Centro de Biociências e Biotecnologia Universidade Estadual do Norte Fluminense- Av. Alberto Lamego 2000 Campos dos Goytacazes RJ 28013-620, Brazil6
Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcântara Gomes UERJ, Blv 28 de Setembro, 87, fundos, 4 andar, Vila Isabel, Rio de Janeiro, RJ 20551-013, Brazil7
Departamento de Informática - Pontifícia Universidade Católica do Rio de Janeiro Rua Marquês de S. Vicente, 225, Rio de Janeiro 22453-900, Brazil8
Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Cx Postal 68011, Rio de Janeiro, RJ 21941-617, Brazil9
Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, Rio de Janeiro, RJ21.949-900, Brazil10
Laboratório de Biologia Molecular, Departamento de Genética e Biologia Molecular, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ 22290-240, Brazil11
Laboratório de Biologia Molecular de Plantas, Instituto de Pesquisas do Jardim, Botânico do Rio de Janeiro, 22460-030 Rio de Janeiro, RJ, Brazil12
...and 2 moreGluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins.
Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins.
Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus.
An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.
| Language: | Undetermined |
|---|---|
| Publisher: | BioMed Central |
| Year: | 2009 |
| Pages: | 450-450 |
| ISSN: | 14712164 |
| Types: | Journal article |
| DOI: | 10.1186/1471-2164-10-450 |
Accessory Region Biological Nitrogen Fixation Biotechnology Comparative Genomic Hybridization DNA, Bacterial Endophytic Bacterium Genetics Genome Island Genome, Bacterial Genomic Islands Genomic Library Gluconacetobacter Horizontal Gene Transfer Molecular Sequence Data Nitrogen Fixation QH426-470 Saccharum Sequence Analysis, DNA Symbiosis TP248.13-248.65
