Journal article
Seroreactivity of Salmonella-infected cattle herds against a fimbrial antigen in comparison with lipopolysaccharide antigens
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark1
National Food Institute, Technical University of Denmark2
Section for Veterinary Epidemiology and public sector consultancy, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark3
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
National Veterinary Institute, Technical University of Denmark5
The IgG seroreaction of Salmonella-infected cattle herds against a fimbrial antigen (SEF14) was compared with that against lipopolysaccharide (LPS) antigens. Sera from 23 dairy herds (n = 205) from an island with no occurrence of salmonellosis, four herds (n = 303) with recent outbreaks of S. dublin and four herds (n = 168) with recent outbreaks of S. typhimurium, were tested in a SEF14-ELISA, S. dublin LPS (0:1, 9, 12) ELISA and S. typhimurium LPS (0:1, 4, 5; 12) ELISA.
At a cut-off OD of 0.5, only one of the animals tested from the salmonellosis-free island showed significant seroreaction against the SEF14 antigen, which was confirmed in a Western-blot analysis. Three out of the four S. dublin-infected herds had several seroreactors in thr SEF14-ELISA, whereas all the four herds were positive in the 0:1, 9, 12-ELISA.
All but two samples (both from the same herd) in the four S. typhimurium-infected herds, positive in the 0:1,4, 5, 12-ELISA, had OD values below 0.5 in the SEF14-ELISA. The results indicate that cattle can produce detectable specific antibodies against fimbrial antigens which may be used for screening of S. dublin-infected herds, particularly in areas with low prevalence of salmonellosis, increasing the predictive value of serology.
Language: | English |
---|---|
Year: | 1996 |
Pages: | 461-467 |
ISSN: | 05147166 , 14390450 and 09311793 |
Types: | Journal article |
DOI: | 10.1111/j.1439-0450.1996.tb00341.x |
ORCIDs: | Hoorfar, Jeffrey |