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Conference paper

Glucose and maltose metabolism in MIG1-disrupted and MAL-constitutive strains of Saccharomyces cerevisiae

From

Department of Biotechnology, Technical University of Denmark1

Department of Chemical and Biochemical Engineering, Technical University of Denmark2

Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark3

The alleviation of glucose control of maltose metabolism brought about by MIG1 disruption was compared to that by MAL overexpression in a haploid Saccharomyces cerevisiae strain. The sugar consumption profiles during cultivation of the wild type, single transformants and a double transformant in a mixed glucose-maltose medium revealed that the MAL-constitutive strains were more alleviated than the single MIG1-disrupted transformant.

While all transformants exhibited higher maximum specific growth rates (0.24-0.25 h(-1)) in glucose-maltose mixtures than the wild type strain (0.20 h(-1)), the MAL-constitutive transformants grew even faster (0.27-0.30 h(-1)) in pure glucose medium than the wild type strain (0.24 h(-1)).

Language: English
Year: 1997
Pages: 287-292
Proceedings: Meeting on Yeast Nutrition and Natural Habitats at the 18th International Specialised Symposium on Yeast (ISSY)
ISSN: 13342606 and 13309862
Types: Conference paper
ORCIDs: Mikkelsen, Jørn Dalgaard

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