Conference paper
Screening the natural habitat: New peptaibiotics from specimens and pure cultures of the fungicolous fungus Hypocrea pulvinata
Fraunhofer Institute for Molecular Biology and Applied Ecology1
Department of Systems Biology, Technical University of Denmark2
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3
Justus Liebig University Giessen4
University of Vienna5
Aalborg University6
Fraunhofer-Gesellschaft7
In order to further investigate the significance of antibiotics for the producing organism(s) in the natural habitat, we screened specimens of the fungicolous fungus Hypocrea pulvinata growing on its natural hosts Piptoporus betulinus and Fomitopsis pinicola1. Using a peptaibiomics approach2, we detected 17-, 18-, 19- (major sequences), and 20-residue peptide sequences in the five specimens analyzed by (U)HPLC/HR-ESI-QqTOF-MS.
Structures of peptaibiotics found were independently confirmed by analyzing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the specimens1. Major, 19-residue peptaibols were assigned as deletion sequences of the trichosporins B3 lacking the Ala/Aib residue in position3.
Our results corroborate that: i) peptaibiotics are, indeed, biosynthesized in the natural habitat, thus, ii) their membrane-perturbing formation of ion channels may support the parasitic life style of a fungicolous fungus.
| Language: | English |
|---|---|
| Year: | 2012 |
| Proceedings: | 32nd EPS Meeting |
| ISSN: | 10991387 and 10752617 |
| Types: | Conference paper |
| ORCIDs: | Thrane, U. and Nielsen, Kristian Fog |
