Journal article
Cytokine expression of skin T-lymphocytes from patients with atopic dermatitis
Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark. nhi_ton@hotmail.com1
We analysed the cytokine profile of skin T cells by establishing 11 T-cell lines from adult patients with moderate-to-severe atopic eczema using T-cell growth factors interleukin-2 and interleukin-4. We compared T-cell lines from lesional skin of atopic dermatitis patients with those from non-atopic skin of patients with other skin diseases, observing that T-cell lines of patients with atopic dermatitis unstimulated cultures expressed a Th1 profile.
After stimulation with anti-CD3 and anti-CD28 monoclonal antibodies, the cytokine expression showed rapid initial upregulation of Th2 followed by a Th1 profile. Furthermore, strong upregulation of interleukin-10 was observed after 24 h stimulation. Our findings suggest that skin T-lymphocytes from atopic dermatitis patients seem to consist of a heterogenous population of Th1 and Th2 or Th0 cells and the results for secreted cytokines indicate that T-cell lines from each inflammatory skin disease showed the corresponding disease-specific original cytokine profile.
Language: | English |
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Publisher: | Medical Journals Sweden AB |
Year: | 2001 |
Pages: | 3-7 |
ISSN: | 16512057 , 00015555 and 00000000 |
Types: | Journal article |
DOI: | 10.1080/00015550117325 |
Adult Antibodies, Monoclonal Base Sequence Biomarkers Biopsy, Needle Cells, Cultured Cytokines Dermatitis, Atopic Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Molecular Sequence Data Probability RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Skin Statistics, Nonparametric Th1 Cells Th2 Cells