Journal article
Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein
An Aspergillus niger strain expressing a red-shifted green fluorescent protein (GFP) in the cytoplasm under the control of the glucoamylase promoter (PgIaA) was characterized with respect to its physiology and morphology. Although xylose acted as a repressor carbon source during batch cultivations, PgIaA-driven GFP expression by the glucoamylase promoter could be demonstrated in xylose-limited continuous cultures.
In these cultivations, the xylose concentration was therefore too low to cause repression. Transient experiments initiated with a maltose pulse did not further induce red-shifted GFP production in xylose-limited continuous cultures. Maltose induction under conditions of xylose repression was microscopically observed and quantified in a flow-through chamber.
Red-shifted GFP was first produced after 5 h induction. Finally the strain was characterized in glucose-limited continuous cultures, and here the area of the mycelium stained with cytoplasmic GFP increased with increasing specific growth rate, indicating that GFP can be used as a marker of cellular activity in this type of cultivation.
Language: | English |
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Publisher: | Microbiology Society |
Year: | 1999 |
Pages: | 729-734 |
ISSN: | 14652080 , 13500872 , 16083237 and 00262617 |
Types: | Journal article |
DOI: | 10.1099/13500872-145-3-729 |