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Journal article

The Influence of Seed-borne N in 15N Isotope Dilution Studies with Legumes The Influence of Seed-borne N in 15N Isotope Dilution Studies with Legumes

By Jensen, Erik Steen1,2,3; Andersen, A. J.; Thomsen, J. D.

From

Bioenergy and Biomass, Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark1

Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark2

Risø National Laboratory for Sustainable Energy, Technical University of Denmark3

The distriution of seed-borne N in shoot and root of pea and field bean was studied using three methods: 1) determination of the N content in shoot and root of plants grown in sand culture without other N sources. 2) 15N isotope dilution in plants grown in Rhizobium-free medium supplied with 15N-labelled nitrate and 3) determination of the 15N-enrichment in shoot and root of plants in which the seed-borne N was labelled with 15N.

The results from the three methods were concordant, showing that from 44 to 50% of the seed-borne N in the two species was located in above-ground plant parts. The effect of corrections for seed-borne N in studies of nitrogen fixation in legumes is discussed.

Language: English
Year: 1985
Pages: 438-443
ISSN: 00015121
Types: Journal article
DOI: 10.1080/00015128509442055

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