Journal article
Architecture of the E.coli 70S ribosome
The 70S ribosome from E.coli was analysed by neutron scattering focusing on the shape and the internal protein-RNA-distribution of the complex. Measurements on selectively deuterated 70S particles and free 30S and 50S subunits applying conventional contrast variation and proton-spin contrast-variation resulted in a total of 42 scattering curves.
Processing the data on the basis of the spherical harmonic technique, a four-phase model for the 70S ribosome could be generated, which describes the shape of the particle as well as the protein- and the RNA-moieties of each subunit at about 35 Angstrom resolution.
Language: | English |
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Year: | 1997 |
Pages: | 199-201 |
ISSN: | 18732135 and 09214526 |
Types: | Journal article |
DOI: | 10.1016/S0921-4526(96)00910-6 |