Journal article
Mass Spectrometry of Underivatized 15-Hydroxyeicosatetraenoic Acid and 15-Hydroxyeicosapentaenoic Acid
A direct mass spectrometric method for 15-hydroxy metabolites of arachidonic acid and of eicosapentaenoic acid is described. 15-Hydroxyeicosanoids have the capacity to inhibit the formation of leukotrienes, potent mediators of inflammation formed by the 5-lipoxygenase pathway of arachidonic acid metabolism and may therefore have anti-inflammatory properties. 15-Hydroxyeicosatetraenoic acid (15-HETE) and 15-hydroxyeicosapentaenoic acid (15-HEPE) were synthesized using soybean lipoxygenase and arachidonic acid and eicosapentaenoic acid as substrates.
These hydroxy fatty acids were then purified by reversed-phase high-performance liquid chromatography. This modified procedure provides rapid synthesis of multimilligram quantities of 15-hydroxyeicosanoids for in vitro and in vivo studies. Electron impact mass spectra of 15-HETE and 15-HEPE could be obtained directly without derivatizations.
Language: | English |
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Year: | 1988 |
Pages: | 459-461 |
ISSN: | 10969888 , 10765174 , 10529306 , 23763868 and 08876134 |
Types: | Journal article |
DOI: | 10.1002/bms.1200170608 |