Journal article
Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei
Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter.
Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 1995 |
Pages: | 100-102 |
Journal subtitle: | Lower Eukaryotes and Organelles |
ISSN: | 14320983 and 01728083 |
Types: | Journal article |
DOI: | 10.1007/BF00313200 |
Ascomycota Aspergillus nidulans Biochemistry, general Cell Biology GUS Gene Expression Regulation Genetic Engineering Glucuronidase Hordeum Life Sciences Microbial Genetics and Genomics Microbiology Plant Sciences Plants Promoter Regions, Genetic Proteomics Recombinant Proteins Time Factors Transformation, Genetic Tubulin particle gun promoter