Journal article
Metabolic flexibility of d-ribose producer strain of Bacillus pumilus under environmental perturbations
The metabolic reaction rate vector is a bridge that links gene and protein expression alterations to the phenotypic endpoint. We present a simple approach for the estimation of flux distribution at key branch points in the metabolic network by using substrate uptake, metabolite secretion rate, and biomass growth rate for transketolase (tkt) deficient Bacillus pumilus ATCC 21951.
We find that the glucose-6-phosphate (G6P) and pseudo catabolic/anabolic branch points are flexible in the d-ribose-producing tkt deficient strain of B. pumilus. The normalized flux through the pentose phosphate pathway (PPP) varied from 1.5 to 86 % under different growth conditions, thereby enabling substantial extracellular accumulation of d-ribose under certain conditions.
Interestingly, the flux through PPP was affected by the extracellular phosphate concentration and dissolved oxygen concentration. This metabolic flexibility may have been the underlying reason for this strain being selected from thousands of others in a screening for d-ribose producers conducted in the 1970s.
Language: | English |
---|---|
Publisher: | Springer-Verlag |
Year: | 2012 |
Pages: | 1227-1243 |
Journal subtitle: | Official Journal of the Society for Industrial Microbiology and Biotechnology |
ISSN: | 14765535 and 13675435 |
Types: | Journal article |
DOI: | 10.1007/s10295-012-1115-z |
Bacillus Biochemistry, general Bioinformatics Biomass Biotechnology Flux analysis Genetic Engineering Inorganic Chemistry Life Sciences Metabolic Networks and Pathways Microbiology Oxygen Pentose Phosphate Pathway Reliability Ribose SC3 Sensitivity Transketolase Transketolase deficient d-Ribose