Journal article
Validation of a serum neutralization test for detection of antibodies specific to cyprinid herpesvirus 3 in infected common and koi carp (Cyprinus carpio)
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail1
Friedrich-Loeffler-Institute2
Instituto Zooprofilattico Sperimentale delle Venezie3
National Veterinary Research Institute4
Section for Virology, National Veterinary Institute, Technical University of Denmark5
National Veterinary Institute, Technical University of Denmark6
Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a serious infective, notifiable disease affecting common carp and varieties. In survivors, infection is generally characterized by a subclinical latency phase with restricted viral replication. The CyHV-3 genome is difficult to detect in such carrier fish that represent a potential source of dissemination if viral reactivation occurs.
In this study, the analytical and diagnostic performance of an alternative serum neutralization (SN) method based on the detection of CyHV-3-specific antibodies was assessed using 151 serum or plasma samples from healthy and naturally or experimentally CyHV-3-infected carp. French CyHV-3 isolate 07/108b was neutralized efficiently by sera from carp infected with European, American and Taiwanese CyHV-3 isolates, but no neutralization was observed using sera specific to other aquatic herpesviruses.
Diagnostic sensitivity, diagnostic specificity and repeatability of 95.9%, 99.0% and 99.3%, respectively, were obtained, as well as a compliance rate of 89.9% in reproducibility testing. Neutralizing antibodies were steadily detected in infected carp subjected to restrictive or permissive temperature variations over more than 25 months post-infection.
The results suggest that this non-lethal diagnostic test could be used in the future to improve the epidemiological surveillance and control of CyHV-3 disease.
Language: | English |
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Year: | 2017 |
Pages: | 687-701 |
ISSN: | 13652761 and 01407775 |
Types: | Journal article |
DOI: | 10.1111/jfd.12550 |
ORCIDs: | Olesen, Niels Jørgen |