Book chapter
Theoretical and experimental comparison of microelectrode sensing configurations for impedimetric cell monitoring
Polytechnic University of Milan1
Department of Micro- and Nanotechnology, Technical University of Denmark2
Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark3
Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark4
A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. These widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10 μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat.
The characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100 kHz vs. 24 kHz) shifting over time. © 2014 Taylor & Francis Group.
Language: | English |
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Year: | 2013 |
Pages: | 75-82 |
Proceedings: | 6th International Workshop on Impedance Spectroscopy |
Journal subtitle: | Measurement, Modeling and Applications |
ISBN: | 0429227574 , 1138001406 , 1306124107 , 1315795671 , 9780429227578 , 9781138001404 , 9781306124102 and 9781315795676 |
Types: | Book chapter |
DOI: | 10.1201/b16063-13 |
ORCIDs: | Heiskanen, Arto , Andresen, Thomas Lars and Emnéus, Jenny |