Book chapter ยท Journal article
Applying fluorescence correlation spectroscopy to investigate peptide-induced membrane disruption
Department of Micro- and Nanotechnology, Technical University of Denmark1
Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark2
Center for Nanomedicine and Theranostics, Centers, Technical University of Denmark3
Department of Chemistry, Technical University of Denmark4
There is considerable interest in understanding the interactions of antimicrobial peptides with phospholipid membranes. Fluorescence correlation spectroscopy (FCS) is a powerful experimental technique that can be used to gain insight into these interactions. Specifically, FCS can be used to quantify leakage of fluorescent molecules of different sizes from large unilamellar lipid vesicles, thereby providing a tool for estimating the size of peptide-induced membrane disruptions.
If fluorescently labeled lipids are incorporated into the membranes of the vesicles, FCS can also be used to obtain information about whether leakage occurs due to localized membrane perturbations or global membrane destabilization. Here, we outline a detailed step-by-step protocol on how to optimally implement an FCS-based leakage assay.
To make the protocol easily accessible to other researchers, it has been supplemented with a number of practical tips and tricks.
Language: | English |
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Publisher: | Springer |
Year: | 2017 |
Pages: | 159-180 |
Series: | Methods in Molecular Biology |
Journal subtitle: | Methods and Protocols |
ISBN: | 1493967355 , 1493967371 , 9781493967353 and 9781493967377 |
ISSN: | 10643745 and 19406029 |
Types: | Book chapter and Journal article |
DOI: | 10.1007/978-1-4939-6737-7_11 |
ORCIDs: | Kristensen, Kasper , Henriksen, Jonas Rosager and Andresen, Thomas Lars |