Journal article
DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS
In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae.
Following optimization of the media 96 isolates of A. pleuropneumonine were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.
Language: | English |
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Year: | 1995 |
Pages: | 191-197 |
ISSN: | 18732542 and 03781135 |
Types: | Journal article |
DOI: | 10.1016/0378-1135(95)00062-F |