Journal article
The structure of the linkage between the O-specific polysaccharide and the core region of the lipopolysaccharide from Salmonella enterica serovar Typhimurium revisited : LPS from Salmonella enterica sv. Typhimurium
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research, Utrecht University, the Netherlands.1
Salmonella enterica sv. Typhimurium strain 1135 possesses smooth(S)-form lipopolysaccharide (LPS). Although the structures of the core region and the O-specific polysaccharide were investigated intensively between the 1960s and the 1980s, the structure of the linkage region between the O-chain and the core was not elucidated unequivocally.
By using modern MS and high-field NMR spectroscopy for analysis of the isolated carbohydrate backbone of the LPS, it has been shown that it is a beta-D-Galp residue that links the first repeating unit of the O-specific polysaccharide to O-4 of the last D-Glcp residue of the core region. Interestingly, this particular D-Galp residue is alpha-linked in all following repeating units.
The data are discussed with regard to the ligation of O-specific polysaccharide and core region during LPS biosynthesis.
Language: | English |
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Year: | 2000 |
Pages: | 2014-2027 |
ISSN: | 14321033 and 00142956 |
Types: | Journal article |
DOI: | 10.1046/j.1432-1327.2000.01205.x |