Journal article
A reduced population of CD103(+)CD11b(+) dendritic cells has a limited impact on oral Salmonella infection
Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.1
Immunology Section, Lund University, Lund, Sweden.2
Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden. Electronic address: mary-jo.wick@immuno.gu.se.3
CD103(+)CD11b(+) dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103(+)CD11b(+) DC and altered T cell differentiation to protein antigen.
The influence of CD103(+)CD11b(+) DC on oral infection with the gastrointestinal pathogen Salmonella, however, is poorly understood and is investigated here. We show that, despite being infected with Salmonella, CD11c-cre.Irf4 mice (called Cre(+) mice) conserve the reduction in CD103(+)CD11b(+) DC observed in naive Cre(+) mice, particularly in the mesenteric lymph nodes (MLN) but also in the siLP at day 3 post infection.
Moreover, Salmonella-infected Cre(+) mice have a similar bacterial burden in intestinal tissues (siLP, MLN and Peyer's patches) as well as the spleen compared to infected Cre(-) controls. The T cell compartment, including the frequency of IFN-γ and IL-17-producing T cells, is not altered in intestinal tissues of Salmonella-infected Cre(+) mice relative to infected Cre(-) controls.
In addition, no difference between infected Cre(+) and Cre(-) mice was observed in either the concentration of IL-6 or IL-17 in whole tissue lysates of siLP, MLN or Peyer's patches or in the serum concentration of Salmonella-specific IgG and IgM. Overall the data suggest that the reduction of CD103(+)CD11b(+) DC in Cre(+) mice has little if any impact on Salmonella burden in infected tissues or eliciting effector functions important in host survival at later stages of the infection.
Language: | English |
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Year: | 2016 |
Pages: | 72-80 |
ISSN: | 18790542 and 01652478 |
Types: | Journal article |
DOI: | 10.1016/j.imlet.2016.05.012 |
Animals Antigens, CD CD103 CD11b Antigen Cell Differentiation Cells, Cultured Cytokines Dendritic Cells Dendritic cells IRF4 Infection Integrin alpha Chains Interferon Regulatory Factors Intestinal Mucosa Intestine, Small Lymphocyte Activation Mesenteric lymph node Mice Mice, Inbred Strains Mice, Knockout Salmonella Salmonella Infections, Animal Salmonella typhimurium T-Lymphocytes alpha E integrins interferon regulatory factor-4