Journal article
Polymer surface functionalities that control human embryoid body cell adhesion revealed by high throughput surface characterization of combinatorial material microarrays
Laboratory of Biophysics and Surface and Analysis, School of Pharmacy, University of Nottingham, Nottingham, UK, NG7 2RD1
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139 USA2
High throughput materials discovery using combinatorial polymer microarrays to screen for new biomaterials with new and improved function is established as a powerful strategy. Here we combine this screening approach with high throughput surface characterisation (HT-SC) to identify surface structure-function relationships.
We explore how this combination can help to identify surface chemical moieties that control protein adsorption and subsequent cellular response. The adhesion of human embryoid body (hEB) cells to a large number (496) of different acrylate polymers synthesized in a microarray format is screened using a high throughput procedure.
To determine the role of the polymer surface properties on hEB cell adhesion, detailed HT-SC of these acrylate polymers is carried out using time of flight secondary ion mass spectrometry (ToF SIMS), x-ray photoelectron spectroscopy (XPS), pico litre drop sessile water contact angle (WCA) measurement and atomic force microscopy (AFM).
A structure-function relationship is identified between the ToF SIMS analysis of the surface chemistry after a fibronectin (Fn) pre-conditioning step and the cell adhesion to each spot using the multivariate analysis technique partial least squares (PLS) regression. Secondary ions indicative of the adsorbed Fn correlate with increased cell adhesion whereas glycol and other functionalities from the polymers are identified that reduce cell adhesion.
Furthermore, a strong relationship between the ToF SIMS spectra of bare polymers and the cell adhesion to each spot is identified using PLS regression. This identifies a role for both the surface chemistry of the bare polymer and the pre-adsorbed Fn, as-represented in the ToF SIMS spectra, in controlling cellular adhesion.
In contrast, no relationship is found between cell adhesion and wettability, surface roughness, elemental or functional surface composition. The correlation between ToF SIMS data of the surfaces and the cell adhesion demonstrates the ability of identifying surface moieties that control protein adsorption and subsequent cell adhesion using ToF SIMS and multivariate analysis.
Language: | English |
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Publisher: | Elsevier BV |
Year: | 2011 |
Pages: | 8827-8838 |
ISSN: | 18785905 and 01429612 |
Types: | Journal article |
DOI: | 10.1016/j.biomaterials.2010.08.028 |
Adsorption Animals Cell Adhesion Cell Count Cell adhesion Combinatorial Chemistry Techniques Elements Embryoid Bodies Fibronectins High throughput High-Throughput Screening Assays Humans Least-Squares Analysis Mice Microarray Microarray Analysis Polymers Principal Component Analysis Protein adsorption Reproducibility of Results Spectrometry, Mass, Secondary Ion Spectrum Analysis, Raman Structure-Activity Relationship Surface Properties Surface chemistry ToF SIMS Wettability