Book chapter · Journal article
The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements
In
Methods in Molecular Biology
—
2020, Volume 2075, pp. 253-264
Abstract
Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation.
Several methods for bioinformatic analysis of mobilome data are also suggested.
| Language: | English |
|---|---|
| Publisher: | Humana Press |
| Year: | 2020 |
| Pages: | 253-264 |
| Series: | Methods in Molecular Biology |
| Journal subtitle: | Methods and Protocols |
| ISBN: | 1493998765 , 1493998773 , 9781493998760 and 9781493998777 |
| ISSN: | 10643745 and 19406029 |
| Types: | Book chapter and Journal article |
| DOI: | 10.1007/978-1-4939-9877-7_18 |
| ORCIDs: | 0000-0001-8300-7758 , 0000-0002-0188-4587 , 0000-0003-0027-1524 and Jørgensen, Tue Sparholt |
