Journal article · Ahead of Print article
A single-host fermentation process for the production of flavor lactones from non-hydroxylated fatty acids
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
Yeast Metabolic Engineering, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Pre-Pilot Plant, Translational Management, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Lactone flavors with fruity, milky, coconut, and other aromas are widely used in the food and fragrance industries. Lactones are produced by chemical synthesis or by biotransformation of plant-sourced hydroxy fatty acids. We established a novel method to produce flavor lactones from abundant non-hydroxylated fatty acids using yeast cell factories.
Oleaginous yeast Yarrowia lipolytica was engineered to perform hydroxylation of fatty acids and chain-shortening via β-oxidation to preferentially twelve or ten carbons. The strains could produce γ-dodecalactone from oleic acid and δ-decalactone from linoleic acid. Through metabolic engineering, the titer was improved 4-fold, and the final strain produced 282 mg/L γ-dodecalactone in a fed-batch bioreactor.
The study paves the way for the production of lactones by fermentation of abundant fatty feedstocks.
| Language: | English |
|---|---|
| Year: | 2020 |
| Pages: | 427-436 |
| ISSN: | 10967184 and 10967176 |
| Types: | Journal article and Ahead of Print article |
| DOI: | 10.1016/j.ymben.2019.08.009 |
| ORCIDs: | Marella, Eko Roy , Dahlin, Jonathan , Dam, Marie Inger , ter Horst, Jolanda , Christensen, Hanne Bjerre and Borodina, Irina |
