Journal article
The plasma membrane proteome of germinating barley embryos
Cereal seed germination involves a complex coordination between different seed tissues. Plasma membranes must play crucial roles in coordination and execution of germination; however, very little is known about seed plasma membrane proteomes due to limited tissue amounts combined with amphiphilicity and low abundance of membrane proteins.
A fraction enriched in plasma membranes was prepared from embryos dissected from 18 h germinated barley seeds using aqueous two-phase partitioning. Reversed-phase chromatography on C-4 resin performed in micro-spin columns with stepwise elution by 2-propanol was used to reduce soluble protein contamination and enrich for hydrophobic proteins.
Sixty-one proteins in 14 SDS-PAGE bands were identified by LC-MS/MS and database searches. The identifications provide new insight into the plasma membrane functions in seed germination.
Language: | English |
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Publisher: | WILEY-VCH Verlag |
Year: | 2009 |
Pages: | 3787-3794 |
ISSN: | 16159861 and 16159853 |
Types: | Journal article |
DOI: | 10.1002/pmic.200800745 |
ORCIDs: | Svensson, Birte |