Journal article
Quorum Sensing Regulation in Aeromonas hydrophila
We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV).
In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop.
Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.
Language: | English |
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Year: | 2010 |
Pages: | 849-857 |
ISSN: | 10898638 and 00222836 |
Types: | Journal article |
DOI: | 10.1016/j.jmb.2010.01.002 |
ORCIDs: | Sams, Thomas , 0000-0002-8003-7414 , 0000-0001-5004-8609 and 0000-0002-1671-2155 |
4-Butyrolactone Aeromonas hydrophila AhyRI protein, Aeromonas Amino Acid Sequence Bacterial Proteins Base Sequence DNA, Bacterial E. coli Escherichia coli Genes, Bacterial Green Fluorescent Proteins Kinetics Michaelis–Menten Models, Biological Molecular Sequence Data N-butyrylhomoserine lactone Promoter Regions, Genetic Quorum Sensing Recombinant Proteins quorum sensing signal molecules