Journal article
Characterization of a novel xylanase from Armillaria gemina and its immobilization onto SiO2 nanoparticles
Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701, Republic of Korea.1
Enhanced catalytic activities of different lignocellulases were obtained from Armillaria gemina under statistically optimized parameters using a jar fermenter. This strain showed maximum xylanase, endoglucanase, cellobiohydrolase, and β-glucosidase activities of 1,270, 146, 34, and 15 U mL(-1), respectively.
Purified A. gemina xylanase (AgXyl) has the highest catalytic efficiency (k (cat)/K (m) = 1,440 mg mL(-1) s(-1)) ever reported for any fungal xylanase, highlighting the significance of the current study. We covalently immobilized the crude xylanase preparation onto functionalized silicon oxide nanoparticles, achieving 117 % immobilization efficiency.
Further immobilization caused a shift in the optimal pH and temperature, along with a fourfold improvement in the half-life of crude AgXyl. Immobilized AgXyl gave 37.8 % higher production of xylooligosaccharides compared to free enzyme. After 17 cycles, the immobilized enzyme retained 92 % of the original activity, demonstrating its potential for the synthesis of xylooligosaccharides in industrial applications.
| Language: | English |
|---|---|
| Publisher: | Springer-Verlag |
| Year: | 2013 |
| Pages: | 1081-1091 |
| ISSN: | 14320614 and 01757598 |
| Types: | Journal article |
| DOI: | 10.1007/s00253-012-4381-9 |
Armillaria Biotechnology Chemistry Chemistry and Materials Science DNA, Fungal Denaturation constant Endo-1,4-beta Xylanases Enthalpy Enzymes, Immobilized Immobilization Microbial Genetics and Genomics Microbiology Molecular Sequence Data Nanoparticles Oligosaccharides Sequence Analysis, DNA SiO2 Silicon Dioxide Xylanase Xylooligosaccharide
