Journal article
New Fluorescent Nanoparticles for Ultrasensitive Detection of Nucleic Acids by Optical Methods
For decades the detection of nucleic acids and their interactions at low abundances has been a challenging task that has thus far been solved by enzymatic target amplification. In this work we aimed at developing efficient tools for amplification-free nucleic acid detection, which resulted in the synthesis of new fluorescent nanoparticles.
Here, the fluorescent nanoparticles were made by simple and inexpensive radical emulsion polymerization of butyl acrylate in the presence of fluorescent dyes and additional functionalization reagents. This provided ultra-bright macrofluorophores of 9-84nm mean diameter, modified with additional alkyne and amino groups for bioconjugation.
By using click and NHS chemistries, the new nanoparticles were attached to target-specific DNA probes that were used in fluorimetry and fluorescence microscopy. Overall, these fluorescent nanoparticles and their oligonucleotide derivatives have higher photostability, brighter fluorescence and hence dramatically lower limits of target detection than the individual organic dyes.
These properties make them useful in approaches directed towards ultrasensitive detection of nucleic acids, in particular for imaging and in vitro diagnostics of DNA.
Language: | English |
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Year: | 2017 |
Pages: | 1599-1603 |
ISSN: | 14394227 and 14397633 |
Types: | Journal article |
DOI: | 10.1002/cbic.201700125 |
ORCIDs: | 0000-0002-9727-2496 , Astakhova, Kira and 0000-0003-4128-9317 |
3-azidopropan-1-amine Acrylates Aminoacridines Azides BUTYL ACRYLATE Benzothiazoles CANCER Carbocyanines Click Chemistry DNA Probes DROPLET DIGITAL PCR Dynamic Light Scattering EMULSION POLYMERIZATION Fluorescent Dyes Fluorometry GENES Limit of Detection MicroRNAs Microscopy, Electron, Transmission Microscopy, Fluorescence PARTICLES PROBES Particle Size Perylene Propylamines Quinolines THERAPY TUMOR Ultraviolet Rays acridine yellow butyl acrylate cyanine dye 5 fluorescent probes n-butyl acrylate nanoparticles nucleic acids polymerization thiazole orange