Journal article
Exploring the Plant–Microbe Interface by Profiling the Surface-Associated Proteins of Barley Grains
Enzyme and Protein Chemistry, Department of Systems Biology1
Agricultural and Environmental Proteomics, Department of Systems Biology2
Cereal grains are colonized by a microbial community that actively interacts with the plant via secretion of various enzymes, hormones, and metabolites. Microorganisms decompose plant tissues by a collection of depolymerizing enzymes, including β-1,4-xylanases, that are in turn inhibited by plant xylanase inhibitors.
To gain insight into the importance of the microbial consortia and their interaction with barley grains, we used a combined gel-based (2-DE coupled to MALDI-TOF-TOF MS) and gel-free (LC–MS/MS) proteomics approach complemented with enzyme activity assays to profile the surface-associated proteins and xylanolytic activities of two barley cultivars.
The surface-associated proteome was dominated by plant proteins with roles in defense and stress-responses, while the relatively less abundant microbial (bacterial and fungal) proteins were involved in cell-wall and polysaccharide degradation and included xylanases. The surface-associated proteomes showed elevated xylanolytic activity and contained several xylanases. Integration of proteomics with enzyme assays is a powerful tool for analysis and characterization of the interaction between microbial consortia and plants in their natural environment.
Language: | English |
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Publisher: | American Chemical Society |
Year: | 2016 |
Pages: | 1151-1167 |
ISSN: | 15353907 and 15353893 |
Types: | Journal article |
DOI: | 10.1021/acs.jproteome.5b01042 |