Journal article
Preparation of Barley Storage Protein, Hordein, for Analytical Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
The extraction, reduction, and alkylation of barley hordein for routine electrophoresis in sodium dodecyl sulfate-polyacrylamide gels were studied to set up a simple preparation procedure giving well-resolved bands in the electrophoresis gel. Hordein was extracted from single crushed seeds or flour by aqueous 50% propan-2-ol containing a Tris-borate buffer, pH 8.6.
The presence of the buffer facilitates the consecutive complete reduction of the extracted protein in the alcohol. Reduction and alkylation in the buffer containing propan-2-ol give sharper bands in the electrophoresis than reduction in a buffer without propan-2-ol but containing sodium dodecyl sulfate.
Language: | English |
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Year: | 1981 |
Pages: | 61-66 |
ISSN: | 10960309 and 00032697 |
Types: | Journal article |
DOI: | 10.1016/0003-2697(81)90523-6 |