Journal article
Analysis of several irdoid and indole precursors of terpenoid indole alkaloids with a single HPLC run
An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 my, 250x4 mm (Merck) with an eluent of 1 % formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min.
In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divarica loganin, tryptophan, and tryptamine accumulated.
Language: | English |
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Year: | 1996 |
Pages: | 278-280 |
ISSN: | 14390221 and 00320943 |
Types: | Journal article |