Journal article
Molecular cloning and characterization of a GH11 endoxylanase from Chaetomium globosum, and its use in enzymatic pretreatment of biomass
Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, South Korea.1
An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temperature of 40 °C and pH of 5.5.
Using oat-spelt xylan, the determined K m, V max, and k cat/K m values were 0.243 mg ml⁻¹, 4,530 U mg⁻¹ protein, and 7,640 ml s⁻¹ mg⁻¹, respectively. A homology model and sequence analysis of XylCg, along with the biochemical properties, confirmed that XylCg belongs to the GH11 family. Rice straw pretreated with XylCg showed 30 % higher conversion yield than the rice straw pretreated with a commercial xylanase.
Although xylanases have been characterized from fungal and bacterial sources, C. globosum XylCg is distinguished from other xylanases by its high catalytic efficiency and its effectiveness in the pretreatment of lignocellulosic biomass.
Language: | English |
---|---|
Publisher: | Springer Berlin Heidelberg |
Year: | 2013 |
Pages: | 7205-14 |
ISSN: | 14320614 and 01757598 |
Types: | Journal article |
DOI: | 10.1007/s00253-012-4577-z |
Avena Biomass Biomedical and Life Sciences Biotechnology Chaetomium Chaetomium globosum Cloning, Molecular Endo-1,4-beta Xylanases Endo-1,4-β-xylanase Enzyme Stability Escherichia coli Gene Expression Homology modeling Hydrogen-Ion Concentration Kinetics Life Sciences Lignin Microbial Genetics and Genomics Microbiology Models, Molecular Oryza Plant Stems Pretreatment Protein Conformation Recombinant Proteins Saccharification Temperature Xylans lignocellulose