Journal article
Identification of 14 new phosphoproteins involved in important plant mitochondrial processes
Protein phosphorylation is a very important posttranslational modification the role of which is practically unexplored in mitochondria. Using two-dimensional gel electrophoresis followed by mass spectrometry, 14 new phosphoproteins are identified in potato tuber mitochondria, all household proteins also present in mammalian and fungal mitochondria.
Seven of the new phosphoproteins are involved in the tricarboxylic acid cycle or associated reactions, four are subunits of respiratory complexes and involved in electron transport, ATP synthesis and protein processing, two are heat shock proteins and one is involved in defence against oxidative stress.
These findings open up entirely new possibilities for the regulation and signal integration of mitochondrial processes.
| Language: | English |
|---|---|
| Year: | 2003 |
| Pages: | 141-146 |
| ISSN: | 18733468 and 00145793 |
| Types: | Journal article |
| DOI: | 10.1016/S0014-5793(03)00250-3 |
1D, one-dimensional 2D, two-dimensional Adenosine Triphosphate BN, Blue Native Citric Acid Cycle Electrophoresis, Gel, Two-Dimensional FDH, formate dehydrogenase HSP, heat shock protein IEF, isoelectric focusing Mass Spectrometry Mitochondrion Phosphoproteins Phosphorylation Plant Proteins Proteomics Respiratory chain SMP, inside-out submitochondrial particles Superoxide dismutase Tricarboxylic acid cycle
