Journal article
Nano-scale structure in membranes in relation to enzyme action - computer simulation vs. experiment
There is increasing theoretical and experimental evidence indicating that small-scale domain structure and dynamical heterogeneity develop in lipid membranes as a consequence of the the underlying phase transitions and the associated density and composition fluctuations. The relevant coherence lengths are in the nano-meter range.
The nano-scale structure is believed to be important for controlling the activity of enzymes, specifically phospholipases, which act at bilayer membranes. We propose here a lattice-gas statistical mechanical model with appropriate dynamics to account for the non-equilibrium action of the enzyme phospholipase A(2) which hydrolyses lipid-bilayer substrates.
The resulting product molecules are assumed to induce local variations in the membrane interfacial pressure. Monte Carlo simulations of the non-equilibrium properties of the model for one-component as well as binary lipid mixtures show that the enzyme activity is modulated by nano-scale lipid-domain formation in the lipid bilayer and lead to a characteristic lag-burst behavior.
The simulations are found to be in semi-quantitative agreement with experimental data.
Language: | English |
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Year: | 2002 |
Pages: | 313-320 |
ISSN: | 13869485 , 00104655 and 18792944 |
Types: | Journal article |
DOI: | 10.1016/S0010-4655(02)00294-1 |
ORCIDs: | 0000-0002-4258-8960 |