Journal article
Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, GU24 0NF, Surrey, UK.1
A fluorogenic RT-PCR (5'-nuclease probe-based) assay using a primer/probe set designed from the internal ribosomal entry site region of the virus genome was developed for the specific detection of all seven serotypes of foot-and-mouth disease (FMD) virus in epithelial suspensions and cell culture virus preparations.
The reverse transcription polymerase chain reaction (RT-PCR) specifically detected FMD virus in sample submissions from the UK 2001 FMD outbreak with greater sensitivity than our conventional RT-PCR procedure and our routine diagnostic procedures of ELISA and virus isolation in cell culture. The fluorogenic RT-PCR provides relatively fast results, enables a quantitative assessment to be made of virus amounts and can handle more samples and/or replicates of samples in a single assay than the conventional RT-PCR procedure.
Therefore it is seen as a valuable tool to complement the routine diagnostic procedures for FMD virus diagnosis.
| Language: | English |
|---|---|
| Year: | 2002 |
| Pages: | 67-80 |
| ISSN: | 01660934 and 18790984 |
| Types: | Journal article |
| DOI: | 10.1016/s0166-0934(02)00081-2 |
| ORCIDs: | Alexandersen, Soren and 0000-0003-1187-4873 |
Animals Cattle Cells, Cultured DNA Probes Diagnosis Disease Outbreaks Enzyme-Linked Immunosorbent Assay Epithelial Cells Fluorescent Dyes Fluorogenic RT-PCR assay Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Foot-and-mouth disease virus Quantitative RT-PCR RNA, Viral Reference Standards Reverse Transcriptase Polymerase Chain Reaction Reverse transcription polymerase chain reaction Serotyping United Kingdom Virus Cultivation
