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Journal article

Weigle Reactivation in Acinetobacter Calcoaceticus

From

Risø National Laboratory, Risø National Laboratory for Sustainable Energy, Technical University of Denmark1

Risø National Laboratory for Sustainable Energy, Technical University of Denmark2

Weigle (W)-reactivation was demonstrated in Acinetobacter calcoaceticus for the UV-irra-diated lysogenic phage P78. The reactivation factor (survival of irradiated phage on irradiated bacteria/ survival on unirradiated bacteria) reached a maximum value of 20. This was obtained at UV-doses giving phage and host survivals of about 5 times 10-6 and 1 times 10-1, respectively.

Intracellular development of W-reactivated P78 was followed by one-step growth experiments. Conditions which allowed maximal W-reactivation also extended the period of phage production and yielded a somewhat reduced burst size.

Language: English
Year: 1982
Pages: 579-581
ISSN: 17511097 and 00318655
Types: Journal article
DOI: 10.1111/j.1751-1097.1982.tb02613.x

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