Journal article
Fully automated microchip system for the detection of quantal exocytosis from single and small ensembles of cells
Bioanalytics Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Polymer Microsystems for Electrophysiology Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Risø National Laboratory for Sustainable Energy, Technical University of Denmark6
A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca2+-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells.
The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to data obtained using conventional carbon fiber microelectrodes.
The detection sensitivity of the device allows for the detection of less than 10 000 dopamine molecules in a quantal release. The distribution of peak rise-time and full width at half maximum was constant during measurement periods of several minutes demonstrating the stability of the MPA modified surface.
| Language: | English |
|---|---|
| Publisher: | The Royal Society of Chemistry |
| Year: | 2008 |
| Pages: | 323-329 |
| ISSN: | 14730189 and 14730197 |
| Types: | Journal article |
| DOI: | 10.1039/B715107A |
| ORCIDs: | Emnéus, Jenny and Taboryski, Rafael Jozef |
3-Mercaptopropionic Acid Animals Calcium Catecholamines Cells, Immobilized Dopamine Electrochemistry Exocytosis Microarray Analysis Microelectrodes Microfluidic Analytical Techniques PC12 Cells Patch-Clamp Techniques Potassium Rats Reproducibility of Results Sensitivity and Specificity Surface Properties Time Factors
