Journal article
Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method
Department of Environmental Engineering, Technical University of Denmark1
Residual Resource Engineering, Department of Environmental Engineering, Technical University of Denmark2
National Food Institute, Technical University of Denmark3
Division of Industrial Food Research, National Food Institute, Technical University of Denmark4
Specific growth rates (SGR) of freshwater algaespecies (Chlorella vulgaris, Auxenochlorella protothecoides,and Chlorella sorokiniana) and the marine speciesNannochloropsis oculata on various nitrogen sources (ammoniumcarbonate, ammonium chloride, sodium nitrate, andurea) could be determined by in vivo chlorophyll-a autofluorescence.These preferences could be determined before large pH changes occurred in the media, with no significant difference(P>0.05) between buffered and non-buffered media.
In all algal species, acclimatization was observed with no significant difference (P>0.05) between SGRs of the second and third cultivations. ANOVA of SGRs in the acclimatized second and third cultivations revealed preferences for nitrogen sources among most of the algae; C. vulgaris preferred sodiumnitrate over other nitrogen sources, A. protothecoides adapted to urea after no growth in the first cultivation, and the SGRs of N. oculata showed an aversion for sodium nitrateover other nitrogen sources (P<0.05).
| Language: | English |
|---|---|
| Publisher: | Springer Netherlands |
| Year: | 2015 |
| Pages: | 1415-1423 |
| ISSN: | 15735176 and 09218971 |
| Types: | Journal article |
| DOI: | 10.1007/s10811-014-0468-2 |
| ORCIDs: | De Francisci, Davide , Holdt, Susan Løvstad and Angelidaki, Irini |
