Journal article
Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)
Friedrich-Loeffler-Institute1
The Hebrew University-Hadassah Medical School2
Cefas Weymouth Laboratory3
Fish Health Service Saxony4
Fish Health Service Thuringia5
West Pomeranian University of Technology6
Chinese academies of Fishery Sciences7
National Veterinary Research Instiute8
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail9
National Veterinary Institute, Technical University of Denmark10
Fish Diseases, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark11
GeneReach Biotechnology Corporation12
Chinese Academy of Fishery Sciences13
University of Stirling14
...and 4 moreKoi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV.
Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels.
Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.
| Language: | English |
|---|---|
| Year: | 2017 |
| Pages: | 1511-1527 |
| ISSN: | 13652761 and 01407775 |
| Types: | Journal article |
| DOI: | 10.1111/jfd.12621 |
| ORCIDs: | Olesen, Niels Jørgen |
