Journal article
Simultaneous determination of ammonia, dimethylamine, trimethylamine and trimethylamine-N-oxide in fish extracts by capillary electrophoresis with indirect UV-detection
Section for Aquatic Lipids and Oxidation, National Institute of Aquatic Resources, Technical University of Denmark1
National Institute of Aquatic Resources, Technical University of Denmark2
Section for Aquatic Process and Product Technology, National Institute of Aquatic Resources, Technical University of Denmark3
A capillary electrophoretic method with indirect UV detection is described for simultaneous determination of ammonia, dimethylamine (DMA), trimethylamine (TMA) and trimethylamine-N- oxide (TMAO) in aqueous extracts of fish, A buffer consisting of 4 mM formic acid, 5 mM copper(II)sulfate and 3 mM crown ether 18-crown-6 enabled separation of the analytes in 5-10 min.
The use of an extended light path capillary technique resulted in a good sensitivity and repeatability. The linear dynamic range, based on a hydrostatic injection at 50 mbar for 2 s, was from the detection limit to at least 2.5 mM. The detection limit for ammonia, DMA, TMA, and TMAO was less than 0.04 mM, corresponding to 2 mg nitrogen per 100 g fish.
As an extra benefit, the method also provided a quantitative determination of potassium, sodium, calcium and magnesium ions. (C) 2002 Elsevier Science Ltd. All rights reserved.
| Language: | English |
|---|---|
| Year: | 2002 |
| Pages: | 509-518 |
| ISSN: | 03088146 and 18737072 |
| Types: | Journal article |
| DOI: | 10.1016/S0308-8146(01)00289-8 |
| ORCIDs: | Jørgensen, Bo |
