Journal article
Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification
Nord University1
National Institute of Aquatic Resources, Technical University of Denmark2
Section for Marine Ecology and Oceanography, National Institute of Aquatic Resources, Technical University of Denmark3
Plymouth Marine Laboratory4
Continuous Plankton Recorder Survey5
Aarhus University6
University of Connecticut7
Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids.
Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae
Language: | English |
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Publisher: | Wiley |
Year: | 2014 |
Pages: | 1072-1079 |
ISSN: | 17550998 and 1755098x |
Types: | Journal article |
DOI: | 10.1111/1755-0998.12241 |
ORCIDs: | Nielsen, Torkel Gissel , 0000-0003-0658-7183 and 0000-0002-7640-3514 |