Journal article
Design and Validation of Conditional Ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05 : Development of New Conditional Ligands
We designed conditional ligands restricted to HLA-B*08:01, 2B*35:01, and 2B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry.
These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.
Language: | English |
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Publisher: | Wiley |
Year: | 2015 |
Pages: | 967-975 |
ISSN: | 15524930 , 10467386 and 15524922 |
Types: | Journal article |
DOI: | 10.1002/cyto.a.22689 |
ORCIDs: | Frøsig, Thomas Mørch , Hadrup, Sine Reker and 0000-0002-9451-6037 |