Book chapter · Journal article
Solid-Phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence
In
Methods in Molecular Biology
—
2020, Volume 2063, pp. 37-44
Abstract
We report a straightforward protocol for the detection of mutated DNA extracted from cancer cells. The assay combines a step-wise solid-phase hybridization and a readout by fluorescence emission. We detect a single-nucleotide polymorphism in two human oncogenes, BRAF and EGFR, and reach a limit of the detection of 300 pM by conventional fluorometry.
The protocol described herein may be used as a foundation for development of automatic optimized assays capable for detection of mutant DNA and RNA in vitro and in cells.
| Language: | English |
|---|---|
| Publisher: | Springer |
| Year: | 2020 |
| Pages: | 37-44 |
| Series: | Methods in Molecular Biology |
| Journal subtitle: | Methods and Protocols |
| ISBN: | 1071601377 , 1071601385 , 9781071601372 and 9781071601389 |
| ISSN: | 19406029 and 10643745 |
| Types: | Book chapter and Journal article |
| DOI: | 10.1007/978-1-0716-0138-9_4 |
| ORCIDs: | Astakhova, Kira |
