Considerations for implementation of novel enzyme-based processes

Biocatalysis is the use of enzymes to catalyze chemical reactions. It is an established synthesisroute in chemical synthesis, alongside conventional chemistry. Biocatalysis is often applied due to excellent regio and stereoselectivity, in addition to its environmentally benign properties. This thesis aims at increasing the potential use of industrial biocatalysis, both in terms of broadening its current use and expanding it to new applications.

This academic study is carried out through two case studies. These two case studies were chosen because they represent each end of the spectra of biocatalytic applications. The first case study is expanding the use of an established biocatalyst. The second case study investigates the potential of a novel biocatalyst.

In addition, the two case studies have very different implementation challenges, impeding current use. Therefore, arguably, the lessons learned from these two case studies justify general conclusions for biocatalysis, irrespective of their application. The work in this thesis therefore contributes, not only to industrial biocatalysis in these two areas, but also increases the understanding of biocatalysis as a whole.