Journal article
Recombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark2
DTU Fermentation Platform, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Imperial College London4
The production of β-carotene has become increasingly interesting within the biotechnological industry due to a rising demand for safer and more natural colorants, nutritional supplements, and antioxidants. A recent study has described the potential of Yarrowia lipolytica as a β-carotene-producing cell factory, reporting the highest titer of recombinant β-carotene produced to date.
Finding the best conditions to maximize production and scaling up the process to full scale, a costly and time-consuming process, it is often a bottleneck in biotechnology. In this work, we explored the benefits of using micro-fermentation equipment to significantly reduce the time spent on design and optimization of bioreaction conditions, especially in the early stages of process development.
In this proof-of-concept study, a β-carotene producing Y. lipolytica strain was tested in micro-fermentations partly to assess the robustness of the cell factory design and partly to perform media optimization. The medium optimization led us to an improvement of up to 50% in the yield of β-carotene production in the best of the conditions.
Overall, the micro-fermentation system had a high degree of reliability in all tests.
Language: | English |
---|---|
Publisher: | Frontiers Media S.A. |
Year: | 2020 |
Pages: | 29 |
ISSN: | 22964185 |
Types: | Journal article |
DOI: | 10.3389/fbioe.2020.00029 |
ORCIDs: | Martinez, José Luis |