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Conference paper

Micromechanical sensors for the measurement of biopolymer degradation

By Keller, Stephan Sylvest1,2,3; Gammelgaard, Lene3,4; Jensen, M P5; Schmid, Silvan1,2,3; Davis, Zachary James2,3,6; Boisen, Anja1,2,3

From

Nanoprobes Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1

NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2

Department of Micro- and Nanotechnology, Technical University of Denmark3

Nanocarbon, Department of Micro- and Nanotechnology, Technical University of Denmark4

Technical University of Denmark5

Dynamic NEMS Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark6

We present microcantilever-based sensors for the characterization of biopolymer degradation by enzymes. Thin films of Poly(L-lactide) (PLLA) were spray-coated onto SU-8 cantilevers with well-known material properties and dimensions. The micromechanical sensors were immersed in solutions of proteinase K to investigate enzymatic degradation of PLLA.

A decrease of the resonance frequency after immersion indicated degradation of the biopolymer coating and allowed the estimation of the degradation rate at a specific enzyme concentration.

Language: English
Publisher: IEEE
Year: 2011
Pages: 457-460
Proceedings: 24th International Conference on Micro Electro Mechanical Systems
ISBN: 1424496322 , 1424496349 , 9781424496327 and 9781424496341
ISSN: 10846999
Types: Conference paper
DOI: 10.1109/MEMSYS.2011.5734460
ORCIDs: Keller, Stephan Sylvest , Schmid, Silvan and Boisen, Anja
Keywords

Biopolymer coating Biopolymer degradation Enzymes Immersion Microcantilever-based sensors Micromechanical sensors POLY(L-lactide) thin films Poteinase K Resonance frequency SU-8 cantilevers Spray coating

Other keywords

Coatings Degradation Frequency measurement Gold Proteins Resonant frequency Young's modulus bioMEMS biochemistry biodegradable materials biological techniques biopolymer coating biopolymer degradation cantilevers enzymes immersion microcantilever-based sensors micromechanical sensors microsensors molecular biophysics polymer films proteinase K resonance frequency spray coating spray coatings

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