Journal article
Serological and genetic characterisation of bovine respiratory syncytial virus (BRSV) indicates that Danish isolates belong to the intermediate subgroup: no evidence of a selective effect on the variability of G protein nucleotide sequence by prior cell culture adaption and passages in cell culture or calves
Virology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark4
Division of Virology, National Veterinary Institute, Technical University of Denmark5
Danish isolates of bovine respiratory syncytial virus (BRSV) were characterised by nucleotide sequencing of the G glycoprotein and by their reactivity with a panel of monoclonal antibodies (MAbs). Among the six Danish isolates, the overall sequence divergence ranged between 0 and 3% at the nucleotide level and between 0 and 5% at the amino acid level.
Sequence divergences of 7-8%, 8-9% and 2-3% (nucleotide) and 9-11%, 12-16% and 4-6% (amino acid) were obtained in the comparison made between the group of Danish isolates and the previously sequenced 391-2USA, 127UK and 220-69Bel isolates, respectively. Phylogenetic analysis showed that the Danish isolates formed three lineages within a separate branch of the phylogenetic tree.
Nevertheless, the Danish isolates were closely related to the 220-69Bel isolate, the prototype of the intermediate antigenic subgroup. The sequencing of the extracellular part of the G gene of additional 11 field BRSV viruses, processed directly from lung samples without prior adaption to cell culture growth. revealed sequence variabilities in the range obtained with the propagated virus.
In addition, several passages in cell culture and in calves had no major impact on the nucleotide sequence of the G protein. These findings indicated that the previously established variabilities of the G protein of RS virus isolates were not attributable to mutations induced during the propagation of the virus.
The reactivity of the Danish isolates with G protein-specific MAbs were similar to that of the 220-69Bel isolate. Furthermore, the sequence of the immunodominant region was completely conserved among the Danish isolates on one side and the 220-69Bel isolate on the other. When combined, these data strongly suggested that the Danish isolates belong to the intermediate subgroup.
Language: | English |
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Year: | 1998 |
Pages: | 265-279 |
ISSN: | 18732542 and 03781135 |
Types: | Journal article |
DOI: | 10.1016/S0378-1135(98)00226-0 |
ORCIDs: | 0000-0002-5039-3178 and Larsen, Lars Erik |
Amino Acid Sequence Animals Antibodies, Monoclonal Antigens, Viral Base Sequence Bovine respiratory syncytial virus Cattle Cattle Diseases Cattle-viruses Cells, Cultured Denmark G protein, Bovine respiratory syncytial virus Genetic Variation Glycoprotein diversity Molecular Sequence Data Open Reading Frames Phylogeny Polymerase Chain Reaction Respiratory Syncytial Virus Infections Respiratory Syncytial Virus, Bovine Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Viral Envelope Proteins