Journal article
atp mutants of Escherichia coli fail to grow on succinate due to a transport deficiency
Escherichia coli atp mutants, which lack a functional Hf-ATPase complex, are capable of growth on glucose but not on succinate or other C-4-dicarboxylates (Suc(-) phenotype). Suc(+) revertants of an atp deletion strain were isolated which were capable of growth on succinate even though they lack the entire Hf-ATPase complex.
Complementation in trans with the yhiF gene suppressed the growth of the Suc(+) mutants on succinate, which implicates the yhiF gene product in the regulation of C-4-dicarboxylate metabolism. Indeed, when the E. coli C-4-dicarboxylate transporter (encoded by the dctA gene) was expressed in trans, the Suc(-) phenotype of the atp deletion strain reverted to Suc(+), which shows that the reason why the E. coli atp mutant is unable to grow aerobically on C-4-dicarboxylates is insufficient transport capacity for these substrates.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 1998 |
Pages: | 5855-5859 |
ISSN: | 10985530 , 00219193 and 10678832 |
Types: | Journal article |
DOI: | 10.1128/JB.180.22.5855-5859.1998 |
ORCIDs: | Jensen, Peter Ruhdal |
Bacterial Proteins Biological Transport Carrier Proteins Chromosome Mapping Culture Media Dicarboxylic Acid Transporters Escherichia coli Gene Deletion Genetic Complementation Test Mutagenesis Phenotype Proton-Translocating ATPases Succinates Transposases dctA protein, Sinorhizobium meliloti