Conference paper
Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)
Biophotonic Imaging, Nano and Bio-physical Systems, Department of Health Technology, Technical University of Denmark1
Biomedical Engineering, Department of Health Technology, Technical University of Denmark2
Department of Health Technology, Technical University of Denmark3
University of St Andrews4
Diode Lasers and LED Systems, Department of Photonics Engineering, Technical University of Denmark5
Department of Photonics Engineering, Technical University of Denmark6
Andrews University7
Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy.
Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.
| Language: | English |
|---|---|
| Publisher: | SPIE - International Society for Optical Engineering |
| Year: | 2020 |
| Proceedings: | SPIE Photonics West BiOS |
| Series: | Proceedings of Spie, the International Society for Optical Engineering |
| ISSN: | 03610748 |
| Types: | Conference paper |
| DOI: | 10.1117/12.2544205 |
| ORCIDs: | Hansen, Anders K. , Marti, Dominik and Andersen, Peter E. |
